Spread of Haitian Variant <i>Vibrio cholerae</i> O1 Causing Cholera Outbreaks in Odisha, India

Cholera posed a significant threat causing outbreaks/epidemics with high morbidity and mortality in Odisha. This study envisages the characterisation of isolated pathogen from two cholera outbreaks reported 2018 2019 Bargarh Rayagada districts Odisha respectively. Vibrio cholerae O1 were following standard techniques. The different virulent drug resistant genes detected by multiplex PCR assays; whereas ctxB genotypes characterised through double mismatch amplification mutation (DMAMA) assay. further sequenced pulse-field gel electrophoresis (PFGE) was done on some selected strains. clinical water isolates Haitian variant (HCT) V. Ogawa biotype El Tor multi strains both places. All positive for virulence genes. antibiotic like dfrA1 (100%), strB (76.9%), intSXT (61.5%) detected. PFGE results exhibited pulsotypes. These due to multidrug HCT which circulating caused So continuous surveillance diarrheal disorders is highly essential prevent future this region.